Collagen Extraction Optimization from the Skin of the Small-Spotted Catshark (S. canicula) by Response Surface Methodology.

María Blanco,Carmen G Sotelo,José Antonio Vázquez,Ricardo I Pérez-Martín

doi:10.3390/md17010040

María Blanco, Carmen G Sotelo + Show 2 more

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https://doi.org/10.3390/md17010040

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Journal: Marine Drugs	Publication Date: Jan 9, 2019
Citations: 51	License type: CC BY 4.0

Affiliation: Instituto de Investigacións Mariñas

Abstract

The small-spotted catshark is one of the most abundant elasmobranchs in the Northeastern Atlantic Ocean. Although its landings are devoted for human consumption, in general this species has low commercial value with high discard rates, reaching 100% in some European fisheries. The reduction of post-harvest losses (discards and by-products) by promotion of a full use of fishing captures is one of the main goals of EU fishing policies. As marine collagens are increasingly used as alternatives to mammalian collagens for cosmetics, tissue engineering, etc., fish skins represent an excellent and abundant source for obtaining this biomolecule. The aim of this study was to analyze the influence of chemical treatment concentration, temperature and time on the extractability of skin collagen from this species. Two experimental designs, one for each of the main stages of the process, were performed by means of Response Surface Methodology (RSM). The combined effect of NaOH concentration, time and temperature on the amount of collagen recovered in the first stage of the collagen extraction procedure was studied. Then, skins treated under optimal NaOH conditions were subjected to a second experimental design, to study the combined effect of AcOH concentration, time and temperature on the collagen recovery by means of yield, amino acid content and SDS-PAGE characterization. Values of independent variables maximizing collagen recovery were 4 °C, 2 h and 0.1 M NaOH (pre-treatment) and 25 °C, 34 h and 1 M AcOH (collagen extraction).

Highlights

The small-spotted catshark (Scyliorhinus canicula) is one of the most abundant elasmobranchs in the Northeastern Atlantic Ocean [1]
Response Surface Methodology (RSM) is a tool that has been previously used for the optimization of collagen extraction conditions from the skin of different fish species [12,13,18,19], none of those studies included all the key optimization parameters influencing the two main steps of the extraction process. This is the first study optimizing the complete process for the extraction of acid-soluble collagen by means of three variables in each optimization stage: temperature, time and chemical treatment (NaOH or AcOH) concentration, from the skin of the small-spotted catshark
(97 kDa); bovine serum albumin (66 kDa); ovalbumin (45 kDa). This is the first study optimizing the complete process for the extraction of acid-soluble collagen by means of three variables from the skin of the small-spotted catshark using response surface methodology

Summary

Introduction

The small-spotted catshark (Scyliorhinus canicula) is one of the most abundant elasmobranchs in the Northeastern Atlantic Ocean [1]. Its landings are sometimes devoted for human consumption (rendering 10% and 16% of fish weight in the form of skin and viscera by-products respectively), it has low commercial value and very often is captured as by-catch resulting in a very high discard rate reaching 100% in some European fisheries. The reduction of post-harvest fish losses (discards and by-products) by the promotion of a full use of fishing captures is one of the main purposes of EU fishing policies [2]. The full use of fishing captures includes the transformation of raw materials for the isolation/production of molecules that could be used in a wide variety of applications, which is one of the approaches included in the “blue growth” strategy of the European Commission. One interesting bioactive compound which could be obtained from fish discards is collagen.

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