Collagen catabolism through Coll2-1 and Coll2-1NO2 and myeloperoxidase activity in marathon runners

Yves Henrotin,Alain Bosseloir,Thierry Bury,Alain Labasse,Thierry Franck,Michelle Deberg

doi:10.1186/2193-1801-2-92

Abstract

To determine the influence of marathon on the serum levels of two markers of cartilage degradation, Coll2-1 and its nitrated form, Coll2-1NO2, and of a marker of neutrophils activation, the myeloperoxidase (MPO).Coll2-1, Coll2-1NO2, total and active MPO were measured in 98 marathon runners without joint pain and with an average age of 47 years. Sera were taken at rest right before the departure and within 30 min after the marathon. The subjects were submitted to a questionnaire concerning their physical activity and their life style.The levels of Coll2-1, Coll2-1NO2 and active MPO were not affected by age, body mass index, sex or performance. The levels of total MPO were higher in female than in male (p < 0.05), but were not affected by the other parameters. After the marathon, Coll2-1 and Coll2-1NO2 concentrations were slightly but systematically decreased. The total and active MPO concentrations were increased by 2 to 3-fold in comparison to the pre-marathon values (p < 0.001 for total and active MPO). The active MPO/total MPO ratio was significantly enhanced after the marathon (p < 0.001). The variation of total MPO during the marathon was negatively correlated with the training time per week (r = −0.34; p = 0.009).The serum levels of Coll2-1 and Coll2-1NO2 were slightly decreased by marathon, indicating that intensive running could reduce cartilage catabolism. Furthermore, Coll2-1NO2 was not correlated with the total and active MPO indicating that Coll2-1 nitration did not result of a systemic oxidative phenomenon but reflects local changes.

Highlights

Intensive exercises such as marathon running, induce a systemic inflammatory syndrome characterized by neutrophilia and increased plasmatic concentration of myeloperoxidase (MPO), pro-inflammatory cytokines such as interleukin (IL)-1, -6, -8, tumor necrosis factor (TNF) alpha and granulocyte-colony stimulating factor (G-CSF) (Suzuki et al 2003)
Cartilage biomarkers The levels of Coll2-1 and Coll2-1NO2 measured right before the race were not affected by age, BMI, sex, training time and performance
Neutrophil activation and systemic inflammation markers The pre-marathon levels of total MPO measured right before the race were significantly higher in female [median: 57.70 (

Summary

Introduction

Intensive exercises such as marathon running, induce a systemic inflammatory syndrome characterized by neutrophilia and increased plasmatic concentration of myeloperoxidase (MPO), pro-inflammatory cytokines such as interleukin (IL)-1, -6, -8, tumor necrosis factor (TNF) alpha and granulocyte-colony stimulating factor (G-CSF) (Suzuki et al 2003) They induce neutrophils and cytokines accumulation in damaged muscles (Fielding et al 1993; Hellsten et al 1997; Suzuki et al 2000; Nieman 2000) and subsequently the release of myocellular proteins such as creatine kinase (CK) and myoglobulin into the circulation (Bruunsgaard et al 1997; Suzuki et al 1999; Suzuki et al 2000). Several studies investigating cartilage by magnetic resonance imaging (MRI) have shown that competitive or leisure long-distance runners experienced no major short-term or long-term knee cartilage damages, considering that there was no evidence of pre-existing damage (Lazzarini et al 1997; Colbert et al 2000; Lohman et al 2001; Krampla et al 2008; SchuellerWeidekamm et al 2006)

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