Abstract
The patterns of [ 3H]-NaBH 4-reduced bone collagen cross-links from osteopetrotic chickens were compared with those of age-matched controls. Ratios of the reduced cross-links, dihydroxylysinonorleucine (DHLNL) 1 1 Abbreviations : EDTA, ethylenediaminetetracetic acid, disodium salt; DHLNL, dihydroxylysinonorleucine; DHNL, dihydroxynorleucine; DNA, deoxyribonucleic acid; HLNL, hydroxylysinonorleucine; HNL, hydroxynorleucine; MAV-2(0), myeloblastosis-associated virus-2 (osteo); [ 3H]-NaBH 4, tritiated sodium borohydride; NH 4HCO 3, ammonium bicarbonate; PFU, plaque-forming units. to hydroxylysinonorleucine (HLNL), were dramatically increased in tibia bone samples from osteopetrotic birds compared to values from control birds. In addition, the initial HLNL peak from osteopetrotic bone collagen was heterogeneous, whereas DHLNL from osteopetrotic or normal bone collagen and HLNL from normal bone collagen were homogeneous.
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