Collagen and fibronectin surface modification of nanoporous anodic alumina and macroporous silicon for endothelial cell cultures

P Formentín,Ú Catalán,S Fernández-Castillejo,L F Marsal,R Solà,L Pol

doi:10.1186/s13036-018-0111-x

Abstract

BackgroundThe ability to direct the cellular response by means of biomaterial surface topography is important for biomedical applications. Substrate surface topography has been shown to be an effective cue for the regulation of cellular response. Here, the response of human aortic endothelial cells to nanoporous anodic alumina and macroporous silicon with collagen and fibronectin functionalization has been studied.MethodsConfocal microscopy and scanning electron microscopy were employed to analyse the effects of the material and the porosity on the adhesion, morphology, and proliferation of the cells. Cell spreading and filopodia formation on macro- and nanoporous material was characterized by atomic force microscopy. We have also studied the influence of the protein on the adhesion.ResultsIt was obtained the best results when the material is functionalized with fibronectin, regarding cells adhesion, morphology, and proliferation.ConclusionThese results permit to obtain chemical modified 3D structures for several biotechnology applications such as tissue engineering, organ-on-chip or regenerative medicine.

Highlights

The ability to direct the cellular response by means of biomaterial surface topography is important for biomedical applications
Fabrication and characterization of MacroPSi and Nanoporous anodic alumina (NAA) substrates To study the cellular response on different porous materials and on different topography, MacroPSi substrates and NAA samples were fabricated
In this study, macro- and nanoporous surfaces bio-activated with Col and Fn were prepared in order to analyse the effect of the surface topography on the cell behaviour

Summary

Introduction

The ability to direct the cellular response by means of biomaterial surface topography is important for biomedical applications. Substrate surface topography has been shown to be an effective cue for the regulation of cellular response. The response of human aortic endothelial cells to nanoporous anodic alumina and macroporous silicon with collagen and fibronectin functionalization has been studied. The macro- or nanostructures on these materials cause effects on cell behaviors, which could be manipulated via tuning the biophysical properties of the structures. PSi is fabricated by means of anodization of monocrystalline wafers and degrades into orthosilicic acid when in contact with an aqueous environment, which is the bioavailable form of silicon [34, 35]. The structural tuneability of the PSi allows a range of pore sizes from microporous to macroporous

Objectives

Methods

Results

Conclusion