Abstract
In this study, we describe an inter-laboratory collaborative ring trial validation of species-specific TaqMan real-time PCR assays for the detection of porcine- and chicken-derived materials in meat products. We comprehensively evaluated the performance of these assays in different environments and situations. This validation included the participation of thirteen laboratories across Europe and Asia. The results from the thirteen participating laboratories were analyzed to determine the specificity, accuracy, false positive rate, limit of detection (LOD), and probability of detection (POD) of the developed methods. These results indicated that the methods developed to detect porcine- and chicken-derived materials in meat products are robust and repeatable. The false positive and false negative rates were both 0%, and the LOD was determined to be five copies/reaction. The laboratory standard deviation (σL) was 0.30 for both detection methods, indicating that the developed methods are suitable for detection and identification of the porcine- and chicken-derived materials in meat products.
Highlights
The identification and quantification of animal materials derived from different species in food and feedstuffs plays an important role in the supervision of food safety
Accurate detection and identification of animal materials from different species in food and feedstuffs plays an important role in food safety supervision
0.63–0.84 1.18 0.30 3.3 methods for detection and identification of porcine and chicken material will greatly facilitate the regulation of meat products and reduce instances of meat adulteration
Summary
The identification and quantification of animal materials derived from different species in food and feedstuffs plays an important role in the supervision of food safety. Many infectious zoonotic diseases, including swine influenza and avian influenza, can be transmitted to human or animal through consumption of contaminated porcine- or chicken-derived food or feedstuffs [1, 2]. The potential to spread these diseases poses a grave threat to humans and animals health [3], and many countries have banned importing food and feed products that contain porcine or chicken materials from regions affected by these diseases. Incidences of adulteration of food products with porcine or chicken materials can attract significant public attention and can cause problems in various social and religious contexts [4, 5].
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