Collaborative Center for Chemical and Biological Mass Spectrometry

Abstract

Chemical and biological investigators increasingly depend on data from mass spectrometers to answer complex questions in their research. However, high resolution mass spectrometers are difficult to obtain and operate. Alternatively, access to high resolution mass spectrometers can be obtained through academic core facilities, but this involves other obstacles. We have an Agilent Technologies 6520 QTOF LC MS/MS mass spectrometer capable of producing high resolution MS/MS data and providing this data to outside research groups. We use these collaborative opportunities to develop and improve our methods for sample analysis as well as train undergraduate students in sample handling, MS/MS operation and data analysis methods. Currently we are capable of identifying small organic molecules as well as identification of proteins from SDS‐PAGE gels. We are in the initial phases of experimentation with ITRAQ isobaric peptide labeling procedures coupled with 2‐D liquid chromatography. By forming collaborative projects with other research groups we provide our collaborators with MS/MS data that they would be unlikely to otherwise obtain, we educate the next generation of MS/MS scientists and we have the opportunity to further develop methods for unique samples in a collaborative environment. This work was supported by the National Institutes of Health and the National Science Foundation.