Abstract
Penicillin is the most important class of β-lactam antibiotics. The improvement of high-yielding strain has always been the focus of low-cost industrial production. In this study, Penicillium chrysogenum CICC 40654 was pretreated by helium cold plasma, and then used for fermentation to synthesize penicillin. Through single factor test and orthogonal one, the optimal values of pretreatment parameters such as discharge power, discharge time and helium working pressure were discussed. Results showed that the strain pretreated at 130 W and 100 Pa for 35 s had the highest penicillin synthesis (more than 30 μg/g dried mycelium biomass) on fermentation for 3 days, which was about 79.7% higher than that of the control. In summary, green and efficient cold plasma pretreatment enhanced its ability of penicillin accumulation of Penicillium chrysogenum. This study will provide a new methodological basis for improving industrial strains for high-yield biomedicine.
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