Abstract
Development of new, faster methods of biosensor construction is a huge challenge for current science and industry. In this work, biosensor construction was carried out using a new soft plasma polymerization (SPP) method in which a bio-recognition layer of laccase enzyme was polymerized and bonded to a glassy carbon electrode (GCE) substrate under atmospheric pressure with a corona discharge jet. Laccase belongs to the oxidoreductase enzyme group with four copper atoms in its active center. Application of the corona SPP plasma method allows reduction of the time needed for biosensor construction from several hours to minutes. The presented work includes optimization of the laccase bio-recognition layer deposition time, structural studies of the deposited laccase layer, as well as study of the fabricated biosensor applicability for the determination of Rutin in real pharmaceutical samples. This method produces a biosensor with two linear ranges from 0.3 μmol/dm3 to 0.5 μmol/dm3 and from 0.8 μmol/dm3 to 16 μmol/dm3 of Rutin concentration. Results shown in this work indicate that application of the one-step, corona SPP method enables biosensor construction with comparable analytical parameters to biosensors fabricated by conventional, multi-step, wet methods.
Highlights
Biosensors are increasingly popular in the quantitative determination of many chemical compounds and heavy metals
This paper presents a new corona soft plasma polymerization (SPP) method of biosensor construction
The innovative corona SPP method presented in this work is a one-step, environmentally friendly method allowing deposition of the bio-recognition layer without using any additional chemicals
Summary
Biosensors are increasingly popular in the quantitative determination of many chemical compounds and heavy metals They are one of the class of chemical sensors with a bioactive recognition (bio-recognition) layer composed of enzymes, antibodies, DNA or RNA [1]. In the construction of biosensors by the soft plasma polymerization (SPP) method presented in this paper, Laccase enzyme (polyphenoloxidase; EC 1.10.3.2) was used in the form of biological precursor. It belongs to the blue multi-copper-oxidase family and is generated by plants, wood-rotting fungi as well as saprophytic ascomytes [4]. The active center of laccase is composed of three main parts
Sign-in/Register to view highlights & summary Sign-in/Register to access full text options 
Free) 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a call
