Cold isostatic pressing-study on methane production and microbial communities in anaerobic digestion of wheat straw with high-solids

Abstract

In this study, the effect of cold isostatic pressing (CIP) pretreatment on the physicochemical properties and subsequent anaerobic digestion (AD) performance of wheat straw (WS) was explored, which did not have additional chemical reagent over the whole process. The WS was subjected to CIP pretreatment by pressures from 350 to 700 MPa, respectively, while AD was carried out at mesophilic temperature with total solids (TS) 7% and 14%. The mapping indicated that the CIP pretreatment promoted the hydrolysis of WS by destroying the dense structure. The CIP pretreatment changed pH and volatile fatty acids (VFAs) in the AD system. Compared with the control group, the cumulative methane production of CIP pretreatment group with TS 14% increased by 22.07%–56.18%. The acetic acid concentration peaked on 4th day and 14th day with 3943.23 mg/L and 3815.21 mg/L, respectively, in H600, and propionic acid concentration also reached two peaks at the same time with 2014.92 mg/L and 2405.20 mg/L, respectively. It was also observed that the CIP pretreatment maintained the pH in the range of 6.44–7.40, maintaining the stability of the overall system. It was also found that CIP pretreatment increased 9 species of hydrolysis and acidogenic bacteria, and methanogen Methanosarcina, thereby facilitating the hydrolysis and methanogenic stages. Predictive functional profiling comfirmed that CIP pretreatment could promote glycolysis/gluconeogenesis, fatty acid metabolism, and fatty acid degradation, whose relative abundance increased from 1.10% to 1.13%, 0.60%–0.62%, and 0.19%–0.23%. Methanogenic capacity of the system was improved because of CIP pretreatment enhancing the accessibility of microorganisms by disrupting the microstructure of WS. The energy conversion analysis showed that this method without chemical reagent addition is feasible for high-solids anaerobic digestion.