Abstract
Abstract. In the routine antibody screening of 45,000 blood samples, 10 cases of cold‐reacting, autologous anti‐LW were discovered, using a low‐ionic strength‐polybrene Auto‐Analyzer. Two of these were shown to belong to the IgG, and one to the IgM class. The others were suspected to belong to the IgG class. An immune anti‐LW (from a D‐positive, LW‐negative patient) was used for comparison with the cold, ‘naturally‐occurring’ anti‐LW, and further comparisons were made with anti‐D. DEAE fractionation shows a much greater heterogeneity for anti‐LW than for anti‐D, but iso‐electrofocusing of the main IgG fraction shows similar iso‐electric points: 8.8 and 8.9 for anti‐D and anti‐LW, respectively. However, the autologous IgG anti‐LW showed an iso‐electric point of 8.2, representing a different relationship to the bulk of IgG. The ability for immune anti‐LW to discriminate between Rh‐positive and negative cells was far greater with the low‐ionic strength‐polybrene Auto‐Analyzer than with the bromelin‐methyl cellulose method. In manual testing, the DEAE fractions showed such a discriminating ability only with the IgM containing fractions.
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