Abstract

Abstract Caterpillars of the eastern North American moth Pyrrharctia isabella (Lepidoptera: Arctiidae) overwinter under plant debris where they are exposed to subfreezing temperatures. We measured supercooling points (scps), susceptibility to ice formation in body fluids at temperatures above the SCP following contact with external ice (inoculative freezing), freeze tolerance, cryoprotectant accumulation and metabolism of P. isabella caterpillars. They were collected between September 1996 and January 1997 and tested either immediately after collection (field acclimatization) or following 5 C acclimation in the laboratory. Hibernaculum temperatures were previously recorded between late November 1995 and late March 1996. In a dry laboratory environment, caterpillars had scps ranging between −5 to −10 C throughout the study. Moreover, caterpillars survived prolonged (7 days) supercooling at −5 C for 7 days and determinations of their scps, which caused brief internal freezing. We induced inoculative freezin...

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