Abstract
PurposeThe goal of this study was to analyze the effects of cold atmospheric pressure plasma jet operated with Helium (He-CAPPJ) on C. albicans virulence traits and biofilms. The study also aimed to evaluate the cytotoxicity of He-CAPPJ to fibroblasts. MethodsThe effective operational parameters of He-CAPPJ against C. albicans SC 5314 were determined. Sub-inhibitory times of exposure were used to evaluate the effect of He-CAPPJ on morphogenesis (yeast-hyphae transition), adherence to epithelial cells, and exoenzymes production. Cytotoxicity to Vero cells was also assessed by MTT assay. ResultsA promising effect of He-CAPPJ on morphogenesis was observed, with an almost 40 times reduction of the filamentation rate comparing to the non-exposed group. He-CAPPJ was able to reduce C. albicans adherence and to decrease fungal biofilm viability. No effect on exoenzymes production was observed. ConclusionHe-CAPPJ was able to attenuate fungal adherence and morphogenesis with low cytotoxicity to fibroblasts. Therefore, He-CAPPJ has potential to be applied as an adjuvant in the treatment of C. albicans superficial infections.
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