Cold Atmospheric Pressure Microplasma Pipette for Disinfection of Methicillin-Resistant Staphylococcus aureus.

Geunyoung Nam,Sungbo Cho,Yeonsook Jang,Muhwan Kim

doi:10.3390/mi12091103

Abstract

Microbial infections should be controlled and prevented for successful wound healing and tissue regeneration. Various disinfection methods exist that use antibiotics, ultraviolet (UV), heat, radiation, or chemical disinfectants; however, cold atmospheric pressure plasma has exhibited a unique and effective antibacterial ability that is not affected by antibiotic resistance or pain. This study develops a cold atmospheric pressure microplasma pipette (CAPMP) that outputs an Ar plasma plume through a tube with an inner radius of 180 μm for disinfection in a small area. The CAPMP was evaluated using Staphylococcus aureus and methicillin-resistant Staphylococcus aureus diluted in liquid media, spread on solid agar, or covered by dressing gauze. An increase in the treatment time of CAPMP resulted in a decrease in the number of colonies of the grown microorganism (colony forming unit) and an increase in the disinfected area for both bacteria. The disinfection ability of CAPMP was observed when the bacteria were covered with dressing gauze and was dependent on the number of gauze layers.

Highlights

Atmospheric pressure, non-thermal plasma, or cold atmospheric pressure plasma (CAP), which generates plasma ions with unique physical and chemical characteristics, are increasingly being employed for diverse biophysical and medical applications [1,2,3,4,5].A low-temperature plasma exhibits thermal non-equilibrium, and the temperature of its neutrals/ions is lower than that of its electrons; further, the plasma can be employed for the treatment of eukaryotic cells and tissues with the generated reactive species [6]
This study developed a cold atmospheric pressure microplasma pipette (CAPMP) that outputs an Ar plasma plume through a tube with an inner radius of 180 μm for disinfection in a tiny area
The developed CAPMP can be feasibly applied for the disinfection of S. aureus and methicillin-resistant Staphylococcus aureus (MRSA) diluted in liquid media, spread on a solid agar plate, and covered by dressing gauzes

Summary

Introduction

Atmospheric pressure, non-thermal plasma, or cold atmospheric pressure plasma (CAP), which generates plasma ions with unique physical and chemical characteristics, are increasingly being employed for diverse biophysical and medical applications [1,2,3,4,5].A low-temperature plasma exhibits thermal non-equilibrium, and the temperature of its neutrals/ions is lower than that of its electrons; further, the plasma can be employed for the treatment of eukaryotic cells and tissues with the generated reactive species [6]. For better wound healing and therapeutic strategies, CAP has been applied to the surgical or necrotic sites of patients with chronic diseases to effectively reduce microbial communities and prevent infection [13,14,15,16]. 4.0/).

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Conclusion