Abstract
Spontaneous excitatory postsynaptic currents (sEPSC) through AMPA-type channels were recorded on cultured hippocampal pyramidal neurons by means of the whole-cell patch-clamp technique. The protein kinase C (PKC) agonist 4β-phorbol 12-myristate 13-acetate (4β-PMA) produced a long-lasting increase in sEPSC frequency not mimicked by the inactive analogue 4α-PM and blocked by the protein kinase inhibitor staurosporine. The 4β-PMA-induced change in sEPSC frequency occurred without detectable change in [Ca 2+] i. After treatment with the microtubule-disrupting agent colchicine, 4β-PMA caused a small and transient increase in sEPSC frequency. It is concluded that colchicine affects the PKC-induced functional plasticity of nerve cells most likely by disturbing the axonal transport. © 1997 Federation of European Biochemical Societies.
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