Colanic acid biosynthesis in Escherichia coli is dependent on lipopolysaccharide structure and glucose availability

Abstract

Lipopolysaccharide and colanic acid are important forms of exopolysaccharides located on the cell surface of Escherichia coli, but their interrelation with the cell stress response is not well understood. In this study, nine mutant strains with different structures of lipopolysaccharide were constructed from E. coli MG1655 by deletion of a single gene or multiple genes. All mutant strains did not produce colanic acid when grown in LB medium, but six of them could produce colanic acid when grown either in M9 medium in which glucose is the sole carbon source or in LB medium supplemented with glucose. The results indicate that colanic acid production in E. coli is dependent on both lipopolysaccharide structure and glucose availability. However, transcriptional analysis showed that 20 genes related to the colanic acid biosynthesis and the key gene rcsA in the Rcs system were all transcriptionally up-regulated in all of the nine mutant strains no matter they were grown in M9 or LB medium. This suggests that the availability of some nucleotide-sugar precursors shared by the biosynthesis of lipopolysaccharide and colanic acid might play a major role in colanic acid production in E. coli. Lipopolysaccharide pathway might have a huge priority to colanic acid pathway to use the common precursors; therefore, the colanic acid is not produced in MG1655 and the nine mutants when grown in LB medium. In the six mutant strains that can produce colanic acid in the glucose rich media, the common precursors might be abundant because they were not needed for synthesizing the mutant lipopolysaccharide.