Abstract
Background Ewing's sarcoma (ES) is the most common malignant primary bone tumor in children and adolescents. This study is aimed at developing new prognostic markers and building a microRNA-mRNA network in the development of ES. Method GSE80201 and GSE39262 were downloaded from the Gene Expression Omnibus (GEO) database. Bioinformatics analysis was used to download and process data. The coexpression of differentially expressed microRNAs (DEMs) and genes (DEGs) was selected by using R software. The FunRich database was utilized to perform cellular component (CC), molecular function (MF), and biological process (BP) enrichment analysis. Cytoscape and ClueGO were used to perform Kyoto Encyclopedia of Genes and Genomes (KEGG) enrichment analysis and construct the mRNA-microRNA network. The Kaplan-Meier Plotter was used to perform prognosis analysis between the expression level of genes we selected and overall survival (OS) of patients with ES. Univariate analysis and multivariate analysis were carried out to research the prognostic value of identified mRNA expression in ES according to TCGA database. Results By using bioinformatics analysis, 10 DEMs and 5 target mRNAs were identified. Based on the KmPlot software, COL1A2, COL3A1, and TGFBI were significantly related to the OS of patients with ES. High COL3A1 mRNA expression was correlated with distant metastasis, margin status, and poor overall survival of ES. Besides, multivariate analysis indicated that COL3A1 was an independent risk factor for ES patients. Conclusions In conclusion, our results suggest that COL3A1 and its related molecules may be a potential diagnostic and prognostic biomarker for patients with ES.
Highlights
Ewing’s sarcoma (ES) is the most common malignant primary bone tumor in children and adolescents
The results indicated that COL1A2, COL3A1, and TGFBI (Figure 6) were significantly related to the prognosis of patients with ES
We found that the expression level of COL3A1 was not associated with metastasis stage, tumor region, age stage, gender stage, and race stage (p > 0:01) (Table 5)
Summary
Ewing’s sarcoma (ES) is the most common malignant primary bone tumor in children and adolescents. The Kaplan-Meier Plotter was used to perform prognosis analysis between the expression level of genes we selected and overall survival (OS) of patients with ES. Our results suggest that COL3A1 and its related molecules may be a potential diagnostic and prognostic biomarker for patients with ES. With the in-depth study of the occurrence, development, and metastasis mechanism of Ewing sarcoma, the molecular targeted therapy of Ewing sarcoma has gradually become a new research hotspot. At present, it mainly focuses on targeted silencing of EWS/FLI-1 fusion gene, targeted blocking of insulin-like factor receptor, BioMed Research International targeted inhibition of receptor tyrosine kinase, antiangiogenesis, and so on [6]. The study of new therapeutic targets is of great significance for the treatment of Ewing sarcoma
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