Abstract
The insertion sequence IS 6100, belonging to the IS 6 family, normally forms a cointegrate as an end product of transposition. The IS 6100-based minitransposon, Tn 1792, has been developed as a genetic tool to mutagenise antibiotic-producing Streptomyces. Here, we describe resolution of Tn 1792 cointegrates in Streptomyces avermitilis that can facilitate both the initial isolation of Tn 1792 insertion mutants and also the subsequent rescue of Tn 1792-tagged sequences. This is the first reported example of cointegrate resolution for an IS 6-type transposable element. As a result of mutagenesis, several putative genes involved in morphological development and antibiotic production have been isolated.
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