Co-Inoculations with Plant Growth-Promoting Bacteria in the Common Bean to Increase Efficiency of NPK Fertilization

Abstract

Given the hypothesis that co-inoculation with plant growth-promoting bacteria (PGPB) enhances the beneficial effects of Rhizobium tropici with greater mineral nutrition, optimizes biological nitrogen fixation and reduces use of fertilizers in bean plants, the objective of this research was to evaluate the synergistic effects of Rhizobium tropici associated with Azospirillum brasilense, Bacillus subtilis, Pseudomonas fluorescens and their combinations, on increasing the efficiency of NPK fertilization to obtain high winter yields of the (irrigated) common bean in the Cerrado region. The experiment was carried out in the field over two years in a Rhodic Hapludox under a no-till system in Selvíria, Brazil. The experimental design comprised complete randomized blocks with four replications in a 3 × 7 factorial scheme. The treatments consisted of three doses of NPK fertilizer (control—0 kg ha−1 (control); 50% of the recommended dose; 100% of the recommended dose in two parts) and seven doses of inoculation or co-inoculation (control; Rhizobium tropici; R. tropici + Azospirillum brasilense; R. tropici + Bacillus subtilis; R. tropici + Pseudomonas fluorescens; R. tropici + A. brasilense + B. subtilis; R. tropici + A. brasilense + P. fluorescens). The PGPB in the co-inoculations increased the hundred-grain weight, the grain pod−1, the grain plant−1 and the grain yield following the NPK doses. The grain yield of the common bean was increased by co-inoculation with R. tropici + A. brasilense + P. fluorescens without NPK treatments, co-inoculation with R. tropici + P. fluorescens and R. tropici + A. brasilense + B. subtilis with the 50% dose of NPK and co-inoculation with R. tropici + B. subtilis with the recommended dose of NPK fertilizer (100%).