Co-immobilization of cellulase and glucose isomerase by molecular deposition technique

Abstract

Cellulase was immobilized and co-immobilized with glucose isomerase within p-trimethylamine polystyrene beads using a molecular deposition technique. The co-immobilized enzyme system directly converted insoluble cellulose to glucose and fructose in 60:40 molar ratio. The co-immobilized enzyme still retained 50% of its initial activity at 50°C after 4 recycles of 5 hours for each time.