COI-BASED DNA BARCODING OF SELAIS FISH FROM ARUT RIVER, CENTRAL KALIMANTAN, INDONESIA

Abstract

Selais fish belongs to famili Siluridae consisting of 12 genera with 104 properly validated species. The human need for these fish has sharply increased due to the benefits provided especially for consumption. However, morphologically the selais fish are slightly difficult to differentiate among other silurid fish for non-specialist experts. Thus, a DNA barcoding approach using the mitochondrial COI gene as a molecular marker in this study was applied to clarify a taxonomic status and classification species of selais fish from Arut River (Central Kalimantan, Indonesia) and was also to assembly COI database library of the fish from Indonesia. The method used in this study was a PCR method with a pair of universal primers FishF2 and FishR2. Based on COI-based DNA barcoding, the whole samples showed there were no sequence differences (only 1 haplotype) within the population and this confirmed that these fish only consisted of one identical species. Furthermore, phylogenetic analysis (NJ / ML / BI) revealed that selais fish in this study had a close genetic relationship with Ompok hypophthalmus compared to other Ompok groups. This relationship was supported by the genetic distance value not exceeding 3.6% and this evaluated the undetermined naming of the selais fish from Arut River which was previously still unclassifiable.