Cohnella pontilimi sp. nov., isolated from tidal-flat mud.

Abstract

A Gram-positive, aerobic, endospore-forming, rod-shaped bacterial strain, CAU 1483T, was isolated from tidal-flat mud in the Republic of Korea. It grew optimally at 30°C, in a pH 7.0 medium with 2% (w/v) NaCl. Phylogenetic analysis based on the 16S rRNA gene sequence indicated that strain CAU 1483T formed a separate clade within Paenibacillaceae together with members of the genus Cohnella. Strain CAU 1483T exhibited the highest 16S rRNA gene sequence similarity (97.1%) to C. candidum 18JY8-7T. Whole genome of strain CAU 1483T was 4.29Mb in size with a 53.7mol% G + C content, and included 4046 coding sequences and included 4046 coding sequences, some of which associated with stress response. The average nucleotide identity and digital DNA-DNA hybridization similarity between strain CAU 1483T and related members of the genus Cohnella were 71.8-74.9% and 22.6-33.9%, respectively. The major respiratory quinone present in this strain was menaquinone-7. Strain CAU 1483T contained anteiso-C15:0 and iso-C16:0 as the major fatty acids, while its polar lipids consisted of phosphatidylglycerol, phosphatidylethanolamine, diphosphatidylglycerol, lysyl-phosphatidylglycerol, phosphatidylcholine, three unidentified aminophospholipids, two unidentified lipids and an unidentified phospholipid. Peptidoglycan type was A1γ meso-Dpm. On the basis of taxonomic characterization, strain CAU 1483T constitutes a novel species, for which the name Cohnella pontilimi sp. nov. is proposed. The type strain of this novel species is CAU 1483T (= KCTC 43047T = NBRC 113953T).