Cohnella cholangitidis sp. nov., a novel species of the genus Cohnella isolated from a clinical specimen in Korea

Joon Ki Kim,Dae-Won Kim,Min Kyoung Shin,Chi-Hwan Choi,Woo-Kon Lee,Myunghwan Jung,Su Yeon Kim,Kyu Jam Hwang,Young Sill Choi

doi:10.1007/s00203-021-02565-3

Abstract

A Gram-positive, aerobic, rod-shaped bacterium, designated as strain 1605-214T, was isolated from the blood sample of a patient with cholangitis. Based on its 16S rRNA gene sequence, the strain 1605-214T belonged to the genus Cohnella and exhibited 97.9% sequence identity with Cohnella luojiensis DSM 24270T (GQ214052). DNA–DNA hybridization, digital DNA–DNA hybridization, and average nucleotide identity values between the two species were 23% ± 1.9, 21.1%, and 77.2%, respectively. The cellular fatty acids of strain 1605-214T were mainly comprised of anteiso-C15:0 (36.1%), iso-C16:0 (16.5%), and C16:0 (15.1%). The predominant quinone was menaquinone-7; predominant polar lipids were diphosphatidylglycerol, phosphatidylethanolamine, and aminophospholipid-1. The cell wall peptidoglycan of strain 1605-214T contained meso-diaminopimelic acid. DNA G + C content of strain 1605-214T was 50.6 mol%. 5187 genes out of a total of 5413 (94.6%) were assigned putative functions using eggNOG v5.0. Based on genotypic characteristics and genomic sequence analysis results, strain 1605-214T was confirmed to represent a novel species of genus Cohnella, for which the name Cohnella cholangitidis sp. nov., was proposed.

Highlights

Communicated by Erko Stackebrandt.Joon Ki Kim and Chi-Hwan Choi contributed to this work.The first species of the genus Cohnella was described as Cohnella thermotolerans in a report by Kämpfer et al (2006)
We have described strain 1605-214T as a novel species of the genus Cohnella
The strain was grown on a blood agar plate (BAP) (KisanBio, Korea) at 30 °C for 48 h and stored at − 70 °C in 10% glycerol

Summary

Present Address

Division of High‐Risk Pathogens, Korea Disease Control and Prevention Agency (KDCA), Cheongju, Republic of Korea. The first species of the genus Cohnella was described as Cohnella thermotolerans in a report by Kämpfer et al (2006). Most members of Cohnella were isolated from various environments such as soil (Cai et al 2010; Kim et al 2010, 2011), plants (Garcia-Fraile et al 2008), water (Shiratori et al 2010), and industrial materials (Kämpfer et al 2006). Cohnella hongkongensis (Kämpfer et al 2006) and Cohnella massiliensis (Abou Abdallah et al 2019) were isolated from clinical samples (Table S1). We have described strain 1605-214T as a novel species of the genus Cohnella.

Materials and methods

Results and discussion

Conclusion