Abstract
Stem cells based tissue engineering has been one of the potential promising therapies in the research on the repair of tissue diseases including the vocal fold. Decellularized extracellular matrix (DCM) as a promising scaffold has be used widely in tissue engineering; however, it remained to be an important issue in vocal fold regeneration. Here, we applied the hydrogels (hyaluronic acid [HA], HA-collagen [HA-Col], and HA-DCM) to determine the effects of hydrogel on the growth and differentiation of human adipose-derived stem cells (hADSCs) into superficial lamina propria fibroblasts. hADSCs were isolated and characterized by fluorescence-activated cell sorting. The results indicated that HA-DCM hydrogel enhanced cell proliferation and prolonged cell morphology significantly compared to HA and HA-Col hydrogel. Importantly, the differentiation of hADSCs into fibroblasts was also promoted by cogels of HA-Col and HA-DCM significantly. The differentiation of hADSCs towards superficial lamina propria fibroblasts was accelerated by the secretion of HGF, IL-8, and VEGF, the decorin and elastin expression, and the synthesis of chondroitin sulfate significantly. Therefore, the cogel of HA-DCM hydrogel was shown to be outstanding in apparent stimulation of hADSCs proliferation and differentiation to vocal fold fibroblasts through secretion of important growth factors and synthesis of extracellular matrix.
Highlights
Injury to the vocal fold can lead to intractable changes in the composition and distribution of extracellular matrix (ECM) in the lamina propria and cause irreversible vocal scarring through significant fibrosis and deposition of hyperplasia disorderly [1,2,3]
In order to characterize human adipose-derived stem cells (hADSCs), expression of surface markers in cells was analyzed by fluorescence-activated cell sorting (FACS) after 3 passages as in previous methods [25, 26] (Figure 1(a))
We found that the supplementation of Decellularized extracellular matrix (DCM) in hyaluronic acid (HA) hydrogel induced the secretion of HGF, IL-8, and VEGF of hADSCs, which might be involved in the promotion of cell growth, elongation, and differentiation by cogel of HA-DCM
Summary
Injury to the vocal fold can lead to intractable changes in the composition and distribution of extracellular matrix (ECM) in the lamina propria and cause irreversible vocal scarring through significant fibrosis and deposition of hyperplasia disorderly [1,2,3]. With the development of tissue engineering, biomaterials and stem cells based regenerative therapy had been shown to be one of the promising alternative methods for the treatment of vocal fold injury [4, 5]. A number of in vivo applications of ADSCs have been reported in diverse tissue and organ diseases [9,10,11]. The injection of ADSCs derived fibroblast-like cells in the injured vocal fold was proved to heal the vocal fold wound through deposition of ECM compositions in vivo [13]
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