Abstract
Glioma is a primary malignancy in central nervous system. Radiotherapy has been used as one of the standard treatments for glioma for decades. Since radioresistance can reduce the curative efficacy of radiotherapy in glioma, investigating the cause of radioresistance and predicting the tumour radiosensibility appeared particularly important. We previously reported that CFL1 and PGK1 are over-expressed in radioresistant U251 glioma cells. In this study, the level of CFL1 and PGK1 of 113 glioma tissues were measured by ELISA method. The relevance of the expression of these two proteins to radiosensibility was analyzed by mean test and multivariate logistic regression. The survival analysis was carried out in 85 irradiated patients and 105 followed-up patients respectively. The relationship between protein expression and clinical parameters was explored in overall 113 patients, and the correlation between CFL1 and PGK1 were determined as well. Our results showed that the expression of CFL1 and PGK1 were significantly higher (P < 0.001) in radioresistant patients than others. The multivariate Logistic regression demonstrated that the expression of CFL1 (p < 0.001) and PGK1 (p < 0.001) were associated with radioresistance in glioma. The multivariate Cox regression in overall survival suggested that CFL1 level or PGK1 level could be the independent prognosis factor for poor prognosis in 113 glioma patients. In addition, CFL1 expression was positively correlated with PGK1 expression in glioma. The results suggested that as promising indicators, CFL1 and PGK1 could be used to evaluate glioma radiosensibility and prognosis. These two proteins could also be the potential therapeutic targets of glioma.
Highlights
Glioma is the most prevalent and aggressive tumor in central nervous system [1]
We previously reported that CFL1 and Phosphoglycerate kinase 1 (PGK1) are over-expressed in radioresistant U251 glioma cells
The results demonstrated that over-expression of CFL1 and PGK1 were associated with the radioresistance whatever the patients undergo the chemotherapy or not
Summary
Glioma is the most prevalent and aggressive tumor in central nervous system [1]. Its major biological characteristics, including anomalous formation, widespread angiogenesis and infiltrative growth, make it rarely curable. Phosphoglycerate kinase 1 (PGK1) is a major enzyme used in the first ATP-generating step of the glycolytic pathway. It catalyzes the reversible transfer of a phosphate group from 1, 3-diphosphoglycerate to adenosine diphosphate (ADP) producing 3-phosphoglycerate and adenosine triphosphate (ATP) [5, 6]. It has been revealed in the functions associated with up-regulating various malignances [7] such as inhibiting tumour vascularity in tumorigenesis [8] and regulating DNA replication and repair [9]
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