Abstract
Since birds lack the pars intermedia of the pituitary gland, the presence of ACTH-, α-MSH-, and β-MSH-containing cells in the pars distalis of the duck was investigated. By using the peroxidase-anti-peroxidase unlabeled antibody enzyme technique there were found cells reacting positively with antibodies against ACTH 1–24, ACTH 17–39, and α-MSH. Positive reactions could not be obtained with anti-bovine β-MSH serum. The coexistence of α-MSH and ACTH in all cells containing either of the two hormones could be established.
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