Abstract
Coelomic fluid of Lumbricus rubellus (CFL) has attracted interest due to its pharmacological properties, including antitumor effect. Furthermore, it is necessary to evaluate the response to treatment with new cancer therapeutic agents. This study aims to investigate whether the combination of CFL and 5-fluorouracil could reduce FAK protein level and iCa2+ and enhance p21 level. Furthermore, it is necessary to evaluate the response to treatment with new cancer therapeutic agents. After 24 hours of treatment, it was necessary to assess the percentage of apoptosis, FAK, and p21 protein expression by flow cytometry. iCa2+ concentration was measured using immunofluorescence. The combination therapy of CFL with 5-fluorouracil potently suppressed six treatment groups were included in this study. HT-29 cell lines were cultured and divided into six groups: group 1 was treated with vehicle (negative control), groups 2-5 were treated with 5-fluorouracil, groups 3-5 were treated with either CFL 5, 10, or 20 µg/ml immediately after 5-fluorouracil, and group 6 was treated with CFL 20 µg/ml, the progression of colorectal cancer. Combination of CFL and 5-fluorouracil significantly decreased FAK expression (p<0.05), iCa2+ (p<0.05), and increased p21 expression (p<0.05) in HT-29 cells. Our results suggest that CFL has an anticancer potential in colorectal cancer when combined with 5-fluorouracil.
Highlights
Cancer is one of the leading causes of death worldwide
On the basis of the antitumor effect of Coelomic fluid of Lumbricus rubellus (CFL), we examined the therapeutic potential of the CFL in the inhibition of colorectal cancer
As for confirming the improved apoptosis effect of the cotreatment, we assessed the number of apoptosis of HT-29 cells (Figure 1)
Summary
Colorectal cancer is one of the most common malignancies with a high prevalence and low 5-year survival rate [1]. The incidence of colorectal cancer as well as the mortality rate is very high. Due to the high intrinsic resistance of colon cancer to currently available agents, 5FU alone and 5-FU-based combination chemotherapy are not expected to achieve great success [3, 4]. Combination chemotherapy and adjuvant chemotherapy have achieved great success in the treatment of malignant disease. A combination of conventional chemotherapies with new therapies directly targeted against the molecular changes in colon cancer seems to be the most promising strategy
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