Abstract
A set of Trimer phosphoramidites was synthesized covering all 20 amino acid codons. These trimer phosphoramidites can be added during synthesis using standard DNA chemistry. A Reaction Factor (RF) was determined empirically for each trimer to compensate for differences in their relative rate of reaction during coupling. It is therefore possible to introduce an equimolar mix of all 20 amino acid codons, or subsets thereof, at any location within a sequence. By mutating a gene at the codon level rather than at individual bases, it is possible to avoid codon bias, frame-shift mutations and the introduction of stop codons, making Trimer phosphoramidites a highly efficient tool for the exploration of sequence space in proteins.
Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
