Abstract
Here, we report the detection of a novel alphavirus in Australian mosquitoes, provisionally named Yada Yada virus (YYV). Phylogenetic analysis indicated that YYV belongs to the mosquito-specific alphavirus complex. The assembled genome is 11,612 nucleotides in length and encodes two open reading frames.
Highlights
We report the detection of a novel alphavirus in Australian mosquitoes, provisionally named Yada Yada virus (YYV)
Phylogenetic analysis was performed by the creation of alignments of YYV and the structural and nonstructural protein sequences of other alphaviruses using MAFFT v7.429 [13], the removal of ambiguously aligned residues with TrimAl v1.4.1 [14], and maximum likelihood inference using PhyML v3.1 [15] employing the Le-Gascuel (LG) plus gamma distribution model of amino acid substitution and 1,000 bootstrap replicates
The resultant trees were viewed in FigTree v1.4.4 [16]. In both the structural (Fig. 1A) and nonstructural (Fig. 1B) protein trees, YYV was placed in the mosquitospecific alphavirus complex, suggesting that it might have a restricted host range
Summary
We report the detection of a novel alphavirus in Australian mosquitoes, provisionally named Yada Yada virus (YYV). Reads were mapped to assembled contigs using BWA-MEM v0.7.17 r1188 [11]. Three of the 21 traps tested contained contigs that had the strongest BLASTx match to the mosquito-specific Eilat alphavirus (EILV).
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