Abstract
We determined the genomic sequence of a Newcastle disease virus (NDV) line obtained directly from the first NDV isolate, named Herts’33. This strain shared ≤ 90% nucleotide sequence identity with the NDV sequences available in the GenBank database, and formed a distinct branch in a phylogenetic tree. This branch may be considered to represent a separate NDV genotype. Our study indicates that investigation of the genomic sequences of old NDV strains that originated from the early outbreaks of Newcastle disease may alter the phylogenetic grouping of the NDV strains and provide data on the evolution of viral genomes over time.
Highlights
Newcastle disease virus (NDV) belongs to the genus Orthoavulavirus, family Paramyxoviridae, order Mononegavirales
Based on a number of criteria, the most recent genetic characterization classifies strains belonging to the species Avian orthoavulavirus 1 into genotypes I to XXI and numerous subgenotypes within a subset of genotypes [1]
The genome organization was typical for NDV (Fig. 1), and the cleavage site in the F protein was typical for velogenic and mesogenic NDV strains [6, 7]
Summary
Newcastle disease virus (NDV) (species Avian orthoavulavirus 1) belongs to the genus Orthoavulavirus, family Paramyxoviridae, order Mononegavirales. In a 2003 paper, Czeglédi et al [4] revealed that the descendant lines of Herts’33, including the vaccine strain H, differed in their F protein gene coding sequences, and three genetic groups were identified by phylogenetic analysis. It was hypothesized that the phylogenetic lineage that includes the Weybridge-origin Herts’33 strain might be a descendant of the isolate from the first ND case, while the Herts’33 lines that clustered phylogenetically with genotype IV sequences were of unknown origin.
Sign-in/Register to view highlights & summary Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
