Abstract

Methane production from co-digestion of grass with cow dung and silage with cow dung was conducted by a bioaugmentation technique. For self-fermentation, maximum methane yield (MY) of 176.66 and 184.94 mL CH4/g-VSadded were achieved at a ratio of grass to cow dung and silage to cow dung of 1:1, respectively. A higher maximum MY of 179.59 and 208.11 mL CH4/g-VSadded was obtained from co-digestion of grass with cow dung and silage with cow dung bioaugmented with anaerobic sludge at a ratio of 3:1. The solid residue left over after co-digestion at a ratio of 3:1 was pretreated by alkaline plus enzyme before used to produce methane and a maximum MY of 333.63 and 301.38 mL CH4/g-VSadded, respectively, was achieved. Overall power generated from co-digestion of grass with cow dung plus pretreated solid residues and co-digestion of silage with cow dung plus pretreated solid residues were 0.0397 and 0.007 watt, respectively.

Highlights

  • Anaerobic digestion (AD) is a biological process by which organic substrates are degraded and converted to methane (CH4 ) under an anaerobic conditions

  • Results indicated that Rm was greatly improved after the pretreatment of solid residues obtained from a co-digestion of grass with cow dung and silage with cow dung

  • Our results indicated that the enzymatic hydrolysis is much more efficient than the grinding and alkali pretreatment

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Summary

Introduction

Anaerobic digestion (AD) is a biological process by which organic substrates are degraded and converted to methane (CH4 ) under an anaerobic conditions. The AD process is divided into four phases, namely hydrolysis, acidogenesis, acetogenesis, and methanogenesis [1]. Hydrolysis is generally a critical step for the initiation of AD that results in a low methane production when complex substrates such as lignocellulosic materials are used as feedstocks. To resolve this problem, bioaugmentation with an inoculum capable of efficiently producing methane has been conducted. Saravanane et al [3]

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