Abstract
Objective To investigate the mitochondrial function and caspase activity in mouse embryos after human oviductal cell coculture. Design Experimental laboratory study. Setting University gynecology unit. Animal(s) MF-1 (female); BALB/c (male) mice. Intervention(s) Mouse embryos were cocultured with human oviductal cells. Main outcome measure(s) Mitochondrial transmembrane potential (Δψ m) and caspase activity. Result(s) Compared to embryos after coculture in Chatot-Ziomek-Bavister (CZB) medium supplemented with 0.5 mg/mL of BSA (CZB), Δψm of embryos cultured in CZB was significantly lower at the two-cell (CZB, 2.04 ± 0.412; coculture, 4.34 ± 0.563) and morula (CZB, 6.06 ± 0.548; coculture, 7.12 ± 0.568) stages. Cocultured embryos and in vivo developed embryos had comparable Δψm. Caspase activity was not detected in unfragmented cleavage-stage embryos and morula developed in vivo. In vitro cultured morula possessed caspase activity. The activity was significantly reduced in the cocultured morula. Conclusion(s) Human oviductal cells maintained the mitochondria function in terms of mitochondrial transmembrane potential and decreased the caspase activity to improve the development of mouse embryo.
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