Abstract
Facing the crucial issue of high cost in cellulase production from commercial celluloses, inexpensive lignocellulosic materials from agricultural wastes have been attractive. Therefore, several studies have focused on increasing the efficiency of cellulase production by potential microorganisms capable of secreting a high and diversified amount of enzymes using agricultural waste as valuable substrates. Especially, extremophilic bacteria play an important role in biorefinery due to their high value catalytic enzymes that are active even under harsh environmental conditions. Therefore, in this study, we aim to investigate the ability to produce cellulase from coconut-mesocarp of the potential bacterial strain FW2 that was isolated from kitchen food waste in South Korea. This strain was tolerant in a wide range of temperature (−6–75 °C, pH range (4.5–12)) and at high salt concentration up to 35% NaCl. The molecular weight of the purified cellulase produced from strain FW2 was estimated to be 55 kDa. Optimal conditions for the enzyme activity using commercial substrates were found to be 40–50 °C, pH 7.0–7.5, and 0–10% NaCl observed in 920 U/mL of CMCase, 1300 U/mL of Avicelase, and 150 U/mL of FPase. It was achieved in 650 U/mL, 720 U/mL, and 140 U/mL of CMCase, Avicelase, and FPase using coconut-mesocarp, respectively. The results revealed that enzyme production by strain FW2 may have significant commercial values for industry, argo-waste treatment, and other potential applications.
Highlights
The crisis of energy, the combustion of petroleum-based fossil fuels, and the rapid increase in agricultural municipal cellulosic waste have warned us about the picture of living in the future
The phylogenetic bacterial strain FW2 in this study was identified by comparing the sequence of the amplified 16S rRNA gene against sequences deposited in the GenBank database with Accession No MW652625
Based on the 16S rRNA sequences data, the strain FW2 had the highest homology with Bacillus amyloliquefaciens DSM 7T (99.86%) and pairwise similarity with other members shown in Table 1 and Figure 1 [24]
Summary
The crisis of energy, the combustion of petroleum-based fossil fuels, and the rapid increase in agricultural municipal cellulosic waste have warned us about the picture of living in the future. These issues have shifted global efforts to explore and utilize renewable resources for the production of green energy and eco-environmental waste treatment strategies. Cellulase is the second most important enzyme, only behind amylase due to its environmentally friendly and economical biofuels development [4,5,6,7,8,9]. 1960s, cellulase has been used increasingly in food, paper, pulp, textile industries, and pharmaceutical industries.
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