Cocktail of Hyaluronic Acid and Human Amniotic Mesenchymal Cells Effectively Repairs Cartilage Injuries in Sodium Iodoacetate-Induced Osteoarthritis Rats.

Ai-Tong Wang,Ru-Ming Liu,Yu-Jie Zhao,Chang-Yin Yu,Nuo-Xin Wang,Qing-Fang Zhang,Yi Luo,Jian-Hui Xiao

doi:10.3389/fbioe.2020.00087

Ai-Tong Wang, Ru-Ming Liu + Show 6 more

Open Access

https://doi.org/10.3389/fbioe.2020.00087

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Abstract

Osteoarthritis (OA) is one of the most common refractory degenerative articular cartilage diseases. Human amniotic mesenchymal cells (hAMSCs) have emerged as a promising stem cell source for cartilage repair, and hyaluronic acid (HA) has proven to be a versatile regulator for stem cell transplantation. Herein, an effective and straightforward intra-articular injection therapy using a cocktail of hAMSCs and HA was developed to treat knee OA in a rat model. The injured cartilage was remarkably regenerated, yielding results comparable to normal cartilage levels after 56 days of treatment. Both hAMSCs and HA were indispensable organic components in this therapy, in which HA could synergistically enhance the effects of hAMSCs on cartilage repair. The regenerative mechanism was attributed to the fact that the addition of HA comprehensively enhances the activities of hAMSCs, including chondrogenic differentiation, proliferation, colonization, and regenerative modulation. This cocktail paves a new avenue for injection therapy to treat OA, holding the potential to realize rapid clinical translation.

Highlights

Osteoarthritis (OA), a late-stage joint cartilage injury, affects more than a quarter of the adult population around the world, including more than 50 million people in the United States and more than 100 million people in China (Wang and Cao, 2014; Chen et al, 2017)
We evaluated the effects of hyaluronic acid (HA) on chondro-differentiation of Human amniotic mesenchymal cells (hAMSCs)
In the rodent MIA-induced OA model established in the present study, we observed considerable regeneration of cartilage tissues in the combination treatment group (CTG);the curative effect in the combined transplantation group (CTG) group was much better than that in the OA model rats only injected with HA (HG) or hAMSCs (HTG)

Summary

Introduction

Osteoarthritis (OA), a late-stage joint cartilage injury, affects more than a quarter of the adult population around the world, including more than 50 million people in the United States and more than 100 million people in China (Wang and Cao, 2014; Chen et al, 2017). The regeneration of articular cartilage after injury is challenging Routine treatments, such as subchondral drilling, microfracture, periosteum/perichondrium transplantation, and cartilage tissue grafting, relieve the symptoms of the patients to a certain extent, their long-term treatment efficacy is not satisfactory (Yang et al, 2012; Bark et al, 2014; Musumeci et al, 2014; Bhattacharjee et al, 2015). These routine treatments commonly only alleviate pain, ameliorate joint function, and minimize disability but do not regenerate cartilage. There is an enormous clinical need for a more effective and simple treatment of OA

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