Abstract
Two allergenic fractions in crude American cockroach extract have been isolated with Sephadex G-150 SF gel filtration and diethylaminoethylcellulose ion exchange chromatography. The partially purified fractions of Cr-PI and Cr-PII elicit 73% and 93% of patients sensitive to the crude extracts, respectively. Crude extract contains at least 29 antigenic components, as demonstrated by sodium dodecyl sulfate-polyacrylamide gel electrophoresis and crossed immunoelectrophoresis. Eighteen allergenic arcs are identified by crossed radioimmunoelectrophoresis. Electroblotting analysis with sodium dodecyl sulfate-polyacrylamide gel electrophoresis reveals nine allergenic components of molecular weights of 120,000, 110,000, 90,000, 78,000, 72,000, 49,000, 45,000, 29,000, and 26,000 daltons that are able to bind specific IgE. Among these nine principal allergens, two components of molecular weights 78,000 and 72,000 daltons bound 100% of the sera of atopic patients tested. These two proteins are undoubtedly the major allergens of American cockroach extract.
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