Cochlear function in young and adult Fischer 344 rats

Abstract

Fischer 344 (F344) rats are often used as an animal model for investigation of the mechanisms underlying age-related hearing loss. The aim of this study was to assess cochlear function in young (1-month-old) and adult (6-month-old) F344 rats using recording of otoacoustic emissions and auditory brainstem responses (ABRs). The results were compared with control groups of Long Evans (LE) rats of the same ages. The results demonstrate a significant increase in the hearing threshold in F344 rats in comparison with LE rats, expressed mainly at low frequencies (1–2 kHz). In F344 rats, transient evoked otoacoustic emissions were not measurable and distortion product otoacoustic emissions could be detected within a frequency range of 2.4–6.3 kHz. Tympanometric measurements did not reveal any differences in middle ear parameters between F344 and LE rats. The amplitudes of click-evoked ABRs were significantly lower in 6-month-old F344 rats than in LE rats, but other parameters of the ABRs were almost identical in both rat strains. The results demonstrate a significant deficit in low-frequency hearing and altered otoacoustic emissions in both young and adult F344 rats, suggesting a defect of the inner ear sensory epithelium at the apical part of the cochlea.