Abstract
We have developed the bio-electrode measuring the variance of the amount of acetylcholine affected by residual pesticide. The working electrode of the biosensor was made by combination of cobalt phthalocyanine and carbon organic compounds. The biosensors were constructed by screen-printing method. The principle of working electrode is similar to thiocholine sensor. We have fabricated the biosensor using standard screen printing method. Generally, the biosensor made by printing method formed thick film biosensor. When the electrodes were made by electrochemical cells, the generation of current by the addition of enzyme substrate was inhibited by standard solutions of organo-phosphate pesticides. The detection limit of sensor is about 0.5 for carbofuran. We could improve the responsibility of the sensor by controlling the cobalt phthalocyanine and thiocholine concentration ratio. Also we have tested the EPN and Chlorpyrifos pesticides and found that the biosensor is applicable to fast determination of residual pesticides.
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