Cobalt chloride postconditioning as myoprotective therapy in cardiac ischemia-reperfusion.

Abstract

Since damage induced by ischemia-reperfusion (I/R) involves alterations in Ca2+ homeostasis and is reduced by ischemic postconditioning (IP) and that CoCl2 can trigger changes resembling the response to a hypoxic event in normoxia and its blockade on Ca2+ current in heart muscle, our aim was to evaluate CoCl2 as an IP therapeutic tool. Mechanic and energetic parameters of isolated and arterially perfused male Wistar rat heart ventricles were simultaneously analyzed in a model of I/R in which 0.23mmol/L CoCl2 was introduced upon reperfusion and kept or withdrawn after 20min or introduced after 20min of reperfusion. The presence of CoCl2 did not affect diastolic pressure but increased post-ischemic contractile recovery, which peaked at 20min and decreased at the end of reperfusion. This decrease was prevented when CoCl2 was removed at 20min of reperfusion. Total heat release increased throughout reperfusion, while economy increased between 15 and 25min. No effect was observed when CoCl2 was introduced at 20min of reperfusion. In addition, both the area under the contracture curve evoked by 10mmol/L caffeine-36mmol/L Na+ and the contracture tension relaxation rate were higher with CoCl2.Furthermore, CoCl2 decreased the number of arrhythmias during reperfusion and the ventricular damaged area. The presence of CoCl2 in reperfusion induces cardioprotection consistent with the improvement in cellular calcium handling. The use of CoCl2 constitutes a potential cardioprotective tool of clinical relevance.