Abstract
Aim: This study was carried to evaluates of Cobalt chloride (CoCl2) on cardiac mitochondrial function. Methods: H9C2 cardiomyocytes were cultured in medium containing different concentrations of CoCl2. Cell viability, cardiolipin content, mitochondrial function, and mitochondrial oxidative stress were assessed by using Cell Counting Kit-8 and suitable fluorescence kits. Results: The obtained data showed that CoCl2 (200÷400 µM) induced cell death and decreased mitochondrial function of H9C2 cardiomyocytes in dose dependence. Especially, CoCl2 at dose of 300 µM significantly altered values of mitochondrial membrane potential, H2O2 and O2- to 63.79±2.15%, 145.81±5.83% and 143.10±3.07% (of 100% control), respectively. Conclusion: CoCl2 strongly induced cardiomyocyte death via altering mitochondrial function in a dose-dependent manner.
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