Abstract
To develop biocomposite materials with the local sustained-release function of biological factors to promote bone defect repair, coaxial electrospinning technology was performed to prepare a coaxial nanofiber scaffold with super-active platelet lysate (sPL), containing gelatin/PCL/PLLA. The nanofibers exhibited a uniform bead-free round morphology, observed by a scanning electron microscope (SEM), and the core/shell structure was confirmed by a transmission electron microscope (TEM). A mixture of polycaprolactone and sPL encapsulated by hydrophilic gelatin and hydrophobic l-polylactic acid can continuously release bioactive factors for up to 40 days. Encapsulation of sPL resulted in enhanced cell adhesion and proliferation, and sPL loading can increase the osteogenesis of osteoblasts. Besides, in vivo studies demonstrated that sPL-loaded biocomposites promoted the repair of skull defects in rats. Therefore, these results indicate that core–shell nanofibers loaded with sPL can add enormous potential to the clinical application of this scaffold in bone tissue engineering.
Highlights
The treatment of bone destruction disorders, such as large bone defects associated with comminuted fractures or bone tumor resection and nonunion caused by fractures, is a major challenge for orthopedic surgeons
To accurately assess the release kinetics of the growth factors within the super-active platelet lysate (sPL) scaffolds, we evaluated the release of two major growth factors in sPL, vascular endothelial growth factor (VEGF) and insulin-like growth factor (IGF); which were contributed to osteogenesis
In this study, mineralized gelatin/polylactic acid/ polycaprolactone nano ber membranes with different amounts of sPL were successfully prepared by coaxial cospinning technology
Summary
The treatment of bone destruction disorders, such as large bone defects associated with comminuted fractures or bone tumor resection and nonunion caused by fractures, is a major challenge for orthopedic surgeons. Complete and stable reconstruction of bone is an ideal treatment strategy for bone destruction.[1] Tissue engineering scaffolds are the basis for the development of arti cial bone and the key to bone tissue regeneration.[2] The combination of high porosity, exibility and mechanical properties make this type of ber the material of choice for various biomedical applications. Bioscaffolds are a promising drug delivery materials because they can provide supporting scaffolds for growing cells and tissues.[3] In addition, the scaffolds ll the bone defects during bone regeneration.[4]
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