Coating of Polyurethane Scaffold With Arabinogalactan Leads to Increase of Adhesion to Fibroblast Cells by Integrin Molecules Pathway

Abstract

The purpose of this study was to determine the effect of polyurethane blood vein scaffold (PUBVS) and polyurethane blood vein scaffold impregnated with arabinogalactan (PUBVSIAG) on the cell attachment by integrin signaling molecules. After exposure of HFF cells to PUBVS and PUBVSIAG, both cell attachment and cell viability were determined. Also, the expression of integrin signaling genes was evaluated by both Real-time PCR and western blot. This study showed that both PUBVSIAG and arabinogalactan 5% had the same toxicity in all incubation times. Significant differences were shown between the viability of HFF cells when exposed to PUBVS and PUBVSIAG (P<0.001). At all incubation times, PUBVSIAG had higher attached cells compared with PUBVS (P<0.001). In case of PUBVSIAG, the expression of alpha integrin, beta integrin, fibronectin, and laminin was higher than PUBVS (P<0.001). Western blot analysis confirmed the high expression of integrin molecules. In vivo test showed the reconstruction of new micro-vessels.