Abstract
Using a method described by Miller and Hall [(1983) Virology 125, 236-241] for brome mosaic virus, we succeeded in obtaining preparations of an alfalfa mosaic virus specified RNA polymerase that is totally dependent on added RNA. The enzyme makes full-size transcripts on each of the four virion RNAs. Transcription is inhibited by small amounts of coat protein subunits. This is in accordance with a model proposed by Nassuth and Bol [(1983) Virology 124, 75-85] which says that in a late stage of the infection cycle the coat protein is a factor that regulates the minus-strand RNA synthesis in a negative sense.
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