Coaggregation between and among human intestinal and oral bacteria

Abstract

Coaggregation is believed to facilitate the integration of new bacterial species into polymicrobial communities. The aim of this study was to investigate coaggregation between and among human oral and enteric bacteria. Stationary phase cultures of 10 oral and 10 enteric species, chosen on the basis of numerical and ecological significance in their respective environments together with their ease of cultivation, were tested using a quantitative spectrophotometric coaggregation assay in all possible pairwise combinations to provide quantitative coaggregation scores. While 40% of possible partnerships coaggregated strongly for oral strains, strong interactions between oral and gut strains were considerably less common (4% incidence). Coaggregation scores were also weak between members of the intestinal microbiota (7% incidence), apart from Bacteroides fragilis with Clostridium perfringens, and Bifidobacterium adolescentis with C. perfringens. Oral and intestinal bacteria did not strongly interact, apart from B. adolescentis with Fusobacterium nucleatum, Actinomyces naeslundii with C. perfringens and F. nucleatum with Lactobacillus paracasei. Heating and sugar-addition experiments indicated that similar to oral microorganisms, interactions within intestinal bacteria and between intestinal and oral strains were mediated by lectin-carbohydrate interactions.