CO2 Uptake and Electron Transport Rates in Wild-Type and a Starchless Mutant of Nicotiana sylvestris (The Role and Regulation of Starch Synthesis at Saturating CO2 Concentrations).

Abstract

CO2 uptake rate, chlorophyll fluorescence, and 830-nm absorbance were measured in wild-type (wt) Nicotiana sylvestris (Speg. et Comes) and starchless mutant NS 458 leaves at different light intensities and CO2 concentrations. Initial slopes of the relationships between CO2 uptake and light and CO2 were similar, but the maximum rate at CO2 and light saturation was only 30% in the mutant compared with the wt. O2 enhancement of photosynthesis at CO2 and light saturation was relatively much greater in the mutant than in the wt. In 21% O2, the electron transport rate (ETR) calculated from fluorescence peaked near the beginning of the CO2 saturation of photosynthesis. With the further increase of CO2 concentration ETR remained nearly constant or declined a little in the wt but drastically declined in the mutant. Absorbance measurements at 830 nm indicated photosystem I acceptor side reduction in both plants at saturating CO2 and light. Assimilatory charge (postillumination CO2 uptake) measurements indicated trapping of chloroplast inorganic phosphate, supposedly in hexose phosphates, in the mutant. It is concluded that starch synthesis gradually substitutes for photorespiration as electron acceptor with increasing CO2 concentration in the wt but not in the mutant. It is suggested that starch synthesis is co-controlled by the activity of the chloroplast fructose bisphosphatase.