Abstract
Abstract The experimental determination of cytoplasmic and vacuolar pH values is discussed. Despite variation in these values evidence indicates that intracellular pH values are normally regulated within narrow limits. The regulatory mechanisms proposed involve the metabolic consumption of OH& and the active efflux of H +. The evidence for intracellular pH modification in response to CO2 hydration and the production of HCO−3 and H+ is examined. Theoretical calculations and experimental data indicate that CO2 concentrations as high as 5% will lower intracellular pH. Conversely, variation in CO2 levels around atmospheric concentrations is unlikely to perturb intracellular pH. High CO2 levels are found in bulky tissues, and flooded root systems. Evidence is presented that the slow diffusion of dissolved CO2 compared to gaseous CO2 results in its accumulation. It is proposed that the accumulation of respiratory CO2 may reduce intracellular pH values when plant tissues, cells or protoplasts are maintained in a liquid culture medium. Finally, the possible role of dark CO2 fixation and organic acid synthesis in the regulation of intracellular pH is examined.
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