Abstract

In this study, the effects of initial pH (5 − 7), temperature (30 − 38 ◦C) and incubation time (12 − 48 h), as well as yeast extract and free linoleic acid concentrations, respectively (0 – 4 %) and (0 − 400 µL), on the co-production of conjugated linoleic acid (CLA), exopolysaccharides (EPSs) and bacteriocins (BACs) by Lactobacillus acidophilus LA5 and Bifidobacterium animalis subsp. lactis BB12 and their biomass in cheese whey and milk permeate were evaluated. The results showed that biomass, CLA, EPSs and BACs activity ranged Log 0.80 - Log 8.67 g.L-1, 3.08-107.95 µg.mL-1, 107.75-351.92 mg.L-1 and 9.29-14.62 mm, respectively. Yeast extract concentration was the only factor with the positive significant effect on biomass and parabiotic metabolites i.e. its increasing caused to an increase in both of them (p <0.05). The temperature significantly affected the production of biomass and CLA; its increasing resulted in increasing both (p <0.05). The initial pH had significant, but different, effects on EPSs and BACs production (p <0.05) i.e. EPSs and BACs production decreased and increased, respectively, as a result of increased initial pH. Increasing free linoleic acid concentration from 0 up to 400 µL led to increased CLA biosynthesis. Higher biomass, EPSs and BACs are produced in cheese whey, compared with milk permeate, but CLA produced in milk permeate was higher than that obtained in cheese whey. B. animalis BB12 produced more biomass, CLA and EPSs in comparison to L. acidophilus LA5. However, these probiotics had no statistical difference in terms of producing BACs. This work successfully demonstrated the co-production potential of CLA, EPSs and BACs by two commercial probiotics in dairy effluents.

Talk to us

Join us for a 30 min session where you can share your feedback and ask us any queries you have

Schedule a call

Disclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.