Abstract
Omega-3 fatty acids, and specifically docosahexaenoic acid (DHA), are important and essential nutrients for human health. Thraustochytrids are recognised as commercial strains for nutraceuticals production, they are group of marine oleaginous microorganisms capable of co-synthesis of DHA and other valuable carotenoids in their cellular compartment. The present study sought to optimize DHA and squalene production by the thraustochytrid Schizochytrium limacinum SR21. The highest biomass yield (0.46 g/gsubstrate) and lipid productivity (0.239 g/gsubstrate) were observed with 60 g/L of glucose, following cultivation in a bioreactor, with the DHA content to be 67.76% w/wtotal lipids. To reduce costs, cheaper feedstocks and simultaneous production of various value-added products for pharmaceutical or energy use should be attempted. To this end, we replaced pure glucose with organosolv-pretreated spruce hydrolysate and assessed the simultaneous production of DHA and squalene from S. limacinum SR21. After the 72 h of cultivation period in bioreactor, the maximum DHA content was observed to 66.72% w/wtotal lipids that was corresponded to 10.15 g/L of DHA concentration. While the highest DHA productivity was 3.38 ± 0.27 g/L/d and squalene reached a total of 933.72 ± 6.53 mg/L (16.34 ± 1.81 mg/gCDW). In summary, we show that the co-production of DHA and squalene makes S. limacinum SR21 appropriate strain for commercial-scale production of nutraceuticals.
Highlights
Omega-3 fatty acids, and docosahexaenoic acid (DHA), are important and essential nutrients for human health
Crude glycerol has been tested on S. limacinum SR21, the presence of impurities can inhibit growth and lower DHA productivity
We aimed to enhance total lipid concentration and increase DHA content by first optimizing the concentration of artificial seawater in growth medium during batch cultivation of S. limacinum SR21 in Erlenmeyer flasks. This was done by varying the amount of artificial seawater from 25% to 100% (v/v) at a fixed concentration of glucose (30 g/L) along with a C/N ratio of 10 adjusted by yeast extract (Fig. 1A)
Summary
Omega-3 fatty acids, and docosahexaenoic acid (DHA), are important and essential nutrients for human health. The present study sought to optimize DHA and squalene production by the thraustochytrid Schizochytrium limacinum SR21. Cheaper feedstocks and simultaneous production of various value-added products for pharmaceutical or energy use should be attempted To this end, we replaced pure glucose with organosolv-pretreated spruce hydrolysate and assessed the simultaneous production of DHA and squalene from S. limacinum SR21. Conversion of alpha-linolenic acid into omega-3 fatty acids, such as eicosapentaenoic acid (C20:5n−3), docosapentaenoic acid (C22:5n−3), and docosahexaenoic acid (C22:6n−3, DHA) occurs in humans, it happens at a very slow rate[3] and so these fatty acids must be provided by the diet These PUFAs have several health benefits and have been found to be very effective in the preventions and treatment of fetal diseases[4]. Heterotrophic cultivation of S. limacinum SR21 was carried out on saccharified organosolv-pretreated forest biomass, to test a cost-effective and sustainable production of microbial DHA
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