Abstract
BackgroundAutohydrolysis is an extensively investigated pretreatment method due to its environmental friendliness. During autohydrolysis, most xylan from hemicellulose can be converted into xylooligosaccharides (XOS), and cellulose in the autohydrolyzed residues can be transformed into glucose after enzymatic hydrolysis. Both of these are value-added biochemicals in the biorefining process. In this work, paper mulberry (PM), which contains abundant protein, was utilized as a raw material to coproduce XOS and single-cell protein (SCP) through autohydrolysis and fermentation technologies.ResultsThe results showed that 8.3 g of XOS and 1.8 g of amino acids could be recovered in the autohydrolysate (based on 100 g raw material) after autohydrolysis (170 °C, 1 h). Moreover, 5.7 g of low-DP XOS along with 1.8 g of amino acids could be further obtained from the autohydrolysate after hydrolysis with endo-β-1-4-xylanase. In addition, 20.1 g of fermentable monosaccharides was recovered after hydrolyzing the autohydrolyzed PM with cellulase, which can be used to produce 4.8 g of SCP after fermentation with Candida utilis.ConclusionAs a valuable application of PM, a novel process is proposed to coproduce amino acid-rich XOS and SCP through autohydrolysis. The carbohydrate of PM is effectively converted to high value-added products.
Highlights
Autohydrolysis is an extensively investigated pretreatment method due to its environmental friendliness
Most of the xylan in the hemicellulose can be converted into value-added chemicals such as xylooligosaccharides (XOS) and xylose, and the cellulose can be enzymatically hydrolyzed into glucose for further fermentation [7]
Compositional analysis of autohydrolyzed paper mulberry (PM) In this work, the autohydrolysis pretreatment was carried out at 150 °C–190 °C for 1 h to evaluate how PM responded to the increase in autohydrolysis severity
Summary
Autohydrolysis is an extensively investigated pretreatment method due to its environmental friendliness. Most xylan from hemicellulose can be converted into xylooligosaccharides (XOS), and cellulose in the autohydrolyzed residues can be transformed into glucose after enzymatic hydrolysis. Both of these are value-added biochemicals in the biorefining process. Paper mulberry (PM), which contains abundant protein, was utilized as a raw material to coproduce XOS and single-cell protein (SCP) through autohydrolysis and fermentation technologies. Most of the xylan in the hemicellulose can be converted into value-added chemicals such as xylooligosaccharides (XOS) and xylose, and the cellulose can be enzymatically hydrolyzed into glucose for further fermentation [7]. The pretreatment method is the key to the efficient utilization of PM
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