Abstract

The present report provides evidence for co-localization of cytokinins with cell proliferation-associated nuclear proteins. Somatic embryos of Dactylis glomerata in two stages of development are used as a model system comprising both proliferating and initially differentiated cells. Cytokinins are localized using antibodies with marked specificity against isopentenyladenine/adenosine (2iP/2iPA) or zeatin/riboside (Z/ZR). The proliferation-associated nuclear antigen, mitotin, is analysed using a specific monoclonal antibody. The nuclear protein BM28, required for the onset of DNA replication and for cell division, is identified by an affinity-purified polyclonal antibody

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