Co-incorporating leguminous green manure and rice straw drives the synergistic release of carbon and nitrogen, increases hydrolase activities, and changes the composition of main microbial groups

Abstract

Rice straw (RS) was incorporated into paddy soil together with leguminous green manure (e.g., Chinese milk vetch, Astragalus sinicus L., MV) in a mesocosm-scale experiment and the enzyme activity and composition of main microbial groups were measured. Mixing MV and RS caused a synergistic release of residual C and N, leading to a low C/N ratio at the middle and late decomposition stages of the mixture and shortening the time to complete decomposition. Furthermore, the highest abundances of Gram-negative (G−) bacteria and fungi and the highest activities of α- and β-glucosidase, β-cellobiosidase, β-xylosidase, N-acetyl-glucosaminidase, and acid phosphomonoesterase were observed in the residue mixture, especially at the middle and late stages of the experiment. The residue decomposition rate was positively correlated with residual N and mineral N concentrations. Most hydrolases and both oxidase (phenol oxidase and peroxidase) activities, and G− bacterial (especially 18:1 ω5c, Photobacterium) abundance explained the decomposition of mixing residues. In conclusion, co-incorporation of MV and RS could stimulate their decomposition by retaining a relatively low C/N ratio, enhancing G− bacterial abundance and hydrolase activities. Introducing leguminous green manures in paddy fields may be an effective way to accelerate the decomposition of rice straw.