Abstract
Co-Immunoprecipitation assay for HA-tagged proteins from yeast
Highlights
This work is licensed under a Creative Commons Attribution 4.0 International
Collect approximately 5 × 108 cells expressing either untagged or HA-epitope tagged versions of protein to immunoprecipitate from exponentially growing yeast cultures
Lyse the cells by vigorous shaking with glass beads for 3 min at 4°C
Summary
This work is licensed under a Creative Commons Attribution 4.0 International Co-Immunoprecipitation sassy for tagged proteins using yeast cells -Lysis buffer: 50 mM Tris-HCl pH 8, 250 mM NaCl, 5 mM EDTA, 0,1% \(v/v) Triton X-100, and Complete Mini protease inhibitor \(Roche Diagnostics) - HA-probe \(F7) antibody \(SC-7392, Santa Cruz Biotechnology Inc.)
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